Properties
Description
Recombinant Clostridium thermocellum β-mannanase 5A1 produced by Escherichia coli, is responsible for the enzymatic cleavage of β-1,4-mannans and plays a key role in the germination of seeds and microbial hemicellulose degradation.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum β-mannanase 5A1 can be used in industrial and consumer production. It has the ability to hydrolyze 1,4-β-d-mannans, galactomannans, glucomannans, and mannans.
