Properties
Description
Recombinant Clostridium thermocellum pectate lyase 1A produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum pectate lyase 1A can be used for pectin measurement and carbohydrate research and participates in the eliminative cleavage of pectins, polygalacturonic acid (PGA).
