Recombinant Clostridium thermocellum pectate lyase 1A

Synonym: CtPel1A; Polygalacturonic transeliminase; Pectic acid transeliminase; Polygalacturonate lyase; Endopectin methyltranseliminase

Enzyme Commission Number: 4.2.2.2

Product Size

1 mg; 2 mg; 3 mg; 6 mg

Price

Datasheet

MSDS

Properties

Description

Recombinant Clostridium thermocellum pectate lyase 1A produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism.

Expression System

Escherichia coli

Species

Clostridium thermocellum

Concentration

1 mg/mL

Form

Liquid

Purity

≥90%, determined by SDS-PAGE.

Activity

Pectate lyases

Buffer

35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl₂, and 25% (v/v) glycerol

Applications

Recombinant Clostridium thermocellum pectate lyase 1A can be used for pectin measurement and carbohydrate research and participates in the eliminative cleavage of pectins, polygalacturonic acid (PGA).