Properties
Description
Recombinant Clostridium thermocellum pectin acetyl esterase 12A produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin acetyl esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum pectin acetyl esterase 12A can be used in food processing research, pharmaceuticals, and biotechnology. It participates in the de-esterification of 4-nitrophenyl acetate.
