Properties
Description
Recombinant Dickeya dadantii pectin methylesterase 8A produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose. It is produced in Escherichia coli.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin methylesterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Dickeya dadantii pectin methylesterase 8A can be used for catalyzing pectin hydrolysis in the monitoring of biological processes, employing an ultrasonic measurement device. It participates in the de-esterification of methylated pectins.
