Properties
Description
Recombinant Dickeya dadantii rhamnogalacturonan endolyase 4A produced by Escherichia coli, is involved in the cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds in rhamnogalacturonan I domains within pectin.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Activity
Rhamnogalacturonan lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Dickeya dadantii rhamnogalacturonan endolyase 4A can be used for glycobiology research and participates in the eliminative cleavage of rhamnogalacturonan."
