Properties
Description
Recombinant Geobacillus stearothermophilus neopullulanase 13A produced by Escherichia coli, plays a role in the hydrolysis of pullulan to produce panose (6-α-D-glucosylmaltose).
Expression System
Escherichia coli
Species
Geobacillus stearothermophilus
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Geobacillus stearothermophilus neopullulanase 13A can be used in the biotechnology industries for the enzymatic conversion of pullulan into panose. It has the ability to hydrolyze 1,4-α-glucans, pullulan, and β-cyclodextrin (β-CD).
