Properties
Description
Recombinant Klebsiella pneumoniae alginate lyase 7A produced by Escherichia coli, has the E.C. number 4.2.2.-. and specifically acts on alginate.
Expression System
Escherichia coli
Species
Klebsiella pneumoniae
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Klebsiella pneumoniae alginate lyase 7A can be used as a biocatalyst for the saccharification of alginate and participates in the eliminative cleavage of alginates.