Properties
Description
Recombinant Klebsiella pneumoniae feruloyl esterase 1B produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Klebsiella pneumoniae
Purity
≥90%, determined by SDS-PAGE.
Activity
Feruloyl esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Klebsiella pneumoniae feruloyl esterase 1B can be used in research for paper, pulp, food, nutrition, medical and pharmacological industries. It participates in the de-esterification of ferulate crosslinks between hemicelluloses and lignin.