Properties
Description
Recombinant Lachnoclostridium phytofermentans β-D-galactosyl-1,4-L-rhamnose phosphorylase 112A produced by Escherichia coli, belongs to the glycoside hydrolase family 112 (GH112) and is composed of a single domain.
Expression System
Escherichia coli
Species
Lachnoclostridium phytofermentans
Purity
≥90%, determined by SDS-PAGE.
Activity
β-D-Galactosyl-1,4-L-rhamnose phosphorylases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Lachnoclostridium phytofermentans β-D-galactosyl-1,4-L-rhamnose phosphorylase 112A can be used in glycoside synthesis, glycoconjugate production, and carbohydrate modification. It has the ability to hydrolyze β-D-galactosyl-(1-4)-L-rhamnose.
