Properties
Description
Recombinant Lactobacillus casei hexosaminidase 20A produced by Escherichia coli, plays a key role in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides.
Expression System
Escherichia coli
Species
Lactobacillus casei
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Lactobacillus casei hexosaminidase 20A can be used in ELISA, western blot, immunoprecipitation, and SDS-PAGE. It has the ability to hydrolyze lacto-N-triose units (GlcNAc 1-3Gal 1-4Glc).
