Recombinant Lactobacillus crispatus feruloyl esterase 1B

Synonym: LcFae1B; Ferulic acid esterase; Hydroxycinnamoyl esterase; Hemicellulase accessory enzyme; Cinnamoyl ester hydrolase

Enzyme Commission Number: 3.1.1.73

Product Size

0.25 mg; 0.50 mg; 0.75 mg; 1 mg

Price

Datasheet

MSDS

Properties

Description

Recombinant Lactobacillus crispatus feruloyl esterase 1B produced by Escherichia coli, plays a role in cleaving the ester link between the polysaccharide main chain of xylans and monomeric or dimeric ferulates.

Expression System

Escherichia coli

Species

Lactobacillus crispatus

Concentration

0.25 mg/mL

Form

Liquid

Purity

≥90%, determined by SDS-PAGE.

Activity

Feruloyl esterases

Buffer

35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl₂, and 25% (v/v) glycerol

Applications

Recombinant Lactobacillus crispatus feruloyl esterase 1B can be used in research for paper, pulp, food, nutrition, medical, and pharmacological industries. It participates in the de-esterification of ferulate crosslinks between hemicelluloses and lignin.