Properties
Description
Recombinant Lactobacillus gasseri phospho-β-galactosidase 1A produced by Escherichia coli, is a single-domain glycoside hydrolase family 1 (GH1) enzyme.
Expression System
Escherichia coli
Species
Lactobacillus gasseri
Purity
≥90%, determined by SDS-PAGE.
Activity
Phospho-β-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Lactobacillus gasseri phospho-β-galactosidase 1A can be used in carbohydrate and biofuel research. It has the ability to hydrolyze o-nitrophenyl-β-D-galactopyranoside 6-phosphate, o-nitrophenyl-β-D-glucopyranosid 6-phosphate.