Properties
Description
Recombinant Lactobacillus johnsonii feruloyl esterase 1A produced by Escherichia coli, plays a role in cleaving the ester link between the polysaccharide main chain of xylans and monomeric or dimeric ferulates.
Expression System
Escherichia coli
Species
Lactobacillus johnsonii
Purity
≥90%, determined by SDS-PAGE.
Activity
Feruloyl esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Lactobacillus johnsonii feruloyl esterase 1A can be used in research for paper, pulp, food, nutrition, medical, and pharmacological industries. It participates in the de-esterification of ferulate crosslinks between hemicelluloses and lignin.
