Properties
Description
Recombinant Mycobacterium smegmatis N-acetylglucosaminylphosphatidylinositol deacetylase 14A produced by Escherichia coli, serves as a recombinant enzyme produced in Escherichia coli. It hydrolyzes ester linkages found in hydroxycinnamic acids and diferulates present in plant cell walls.
Expression System
Escherichia coli
Species
Mycobacterium smegmatis
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetylglucosamine deacetylases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Mycobacterium smegmatis N-acetylglucosaminylphosphatidylinositol deacetylase 14A can be used for glycobiology research. It participates in the de-esterification of 1-D-myo-inosityl-2-acetamido-2-deoxy-α-D-glucopyranoside (GlcNAc-Ins).
