Properties
Description
Recombinant Paenibacillus sp. glucosylceramidase 3A produced by Escherichia coli, is a single-domain glycoside hydrolase family 3 (GH3) enzyme.
Expression System
Escherichia coli
Species
Paenibacillus sp.
Purity
≥90%, determined by SDS-PAGE.
Activity
Other Activities
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Paenibacillus sp. glucosylceramidase 3A can be used in biotechnology and glycoscience. It has the ability to hydrolyze 4-nitrophenyl-β-D-glucopyranoside (PNPG), glucosylceramide.