Properties
Description
Recombinant Photobacterium profundum exo-β-glucosaminidase 9A produced by Escherichia coli, plays a role in the hydrolysis of chitosan or chitosan oligosaccharides. Its function is to remove successive D-glucosamine residues from the non-reducing termini.
Expression System
Escherichia coli
Species
Photobacterium profundum
Purity
≥90%, determined by SDS-PAGE.
Activity
Glucosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Photobacterium profundum exo-β-glucosaminidase 9A can be used for glycobiology research. It has the ability to hydrolyze cellooligosaccharides, a nonreducing end of chitooligosaccharides.
