Properties
Description
Recombinant Pseudomonas aeruginosa oligoalginate lyase 7A produced by Escherichia coli, is involved in the cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds in rhamnogalacturonan I domains within pectin. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Pseudomonas aeruginosa
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Pseudomonas aeruginosa oligoalginate lyase 7A can be used in the production of bioactive oligosaccharides and participates in the eliminative cleavage of alginates.
