Properties
Description
Recombinant Pseudothermotoga thermarum xylosidase 3A produced by Escherichia coli, is an enzyme with E.C. number 3.2.1.37 and 3.2.1.55, displaying bifunctional activity in xylan and α-L-arabinoside hydrolysis.
Expression System
Escherichia coli
Species
Pseudothermotoga thermarum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Pseudothermotoga thermarum xylosidase 3A can be used in carbohydrate and biofuel research. It has the ability to hydrolyze 4-nitrophenyl-α-L-arabinofuranoside, 4-nitrophenyl-β-D-xylopyranoside (PNPX), xylobiose, xylotetraose, xylotriose.