Properties
Description
Recombinant Pyrococcus furiosus β-glucosidase 1A produced by Escherichia coli, is responsible for hydrolyzing the glycosidic bonds of cellobiose to give rise to glucose, which is crucial for the effective use of cellulose.
Expression System
Escherichia coli
Species
Pyrococcus furiosus
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Pyrococcus furiosus β-glucosidase 1A can be used in carbohydrate research and biofuel industries. It has the ability to hydrolyze 4-nitrophenyl β-d-glucopyranoside (pNPG), cellobiose.
