Properties
Description
Recombinant Pyrococcus horikoshii exo-β-glucosaminidase 35A produced by Escherichia coli, plays a role in the hydrolysis of chitosan or chitosan oligosaccharides. Its function is to remove successive D-glucosamine residues from the non-reducing termini.
Expression System
Escherichia coli
Species
Pyrococcus horikoshii
Purity
≥90%, determined by SDS-PAGE.
Activity
Glucosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Pyrococcus horikoshii exo-β-glucosaminidase 35A can be used for glycobiology research. It has the ability to hydrolyze 1,4-β-xylans, cellobiose, cellotriose, cellulosic carbohydrates, chitin, diacetylchitobiose (GlcNAc2), lactose, laminarin, lichenan, triacetylchitobiose (GlcNAc3).
