Properties
Description
Recombinant Rhizobium sp. malto-oligosyltrehalose trehalohydrolase 13A produced by Escherichia coli, participates in the hydrolysis of the 1,4-α-D-glucosidic linkage in 4-α-D-(1,4-α-D-glucanosyl)(n) trehalose, resulting in the formation of trehalose and 1,4-α-D-glucan.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Rhizobium sp. malto-oligosyltrehalose trehalohydrolase 13A can be used for the preparation of trehalose. It has the ability to hydrolyze 1,4-α-glucans and maltohexaose.
