Properties
Description
Recombinant Ruminiclostridium thermocellum cellulase 5G produced by Escherichia coli, possesses broad exo-1,3/1,6-β-glucanase specificity and endo-β-1,4-glucanase activity.
Expression System
Escherichia coli
Species
Ruminiclostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminiclostridium thermocellum cellulase 5G can be used in research for textile, animal food, pharmaceutical, detergent, and paper processing industries. It has the ability to hydrolyze 2'-chloro-4'-nitrophenyl-β-D-cellobioside, 4-nitrophenyl β-D-cellobioside, and cellulosic carbohydrates.
