Properties
Description
Recombinant Ruminiclostridium thermocellum pectin methylesterase 8A produced by Escherichia coli, functions as a methyl side chain remover for pectins.
Expression System
Escherichia coli
Species
Ruminiclostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin methylesterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminiclostridium thermocellum pectin methylesterase 8A can be used for catalyzing pectin hydrolysis in monitoring biological processes, employing an ultrasonic measurement device. It participates in the de-esterification of pectins.
