Properties
Description
Recombinant Ruminococcus albus β-mannooligosaccharide phosphorylase 130A produced by Escherichia coli, catalyzes the reversible phosphorolysis of 1,4-β-mannooligosaccharide with a minimum size of three monomers.
Expression System
Escherichia coli
Species
Ruminococcus albus
Purity
≥90%, determined by SDS-PAGE.
Activity
Other Activities
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminococcus albus β-mannooligosaccharide phosphorylase 130A can be used in research for food additives, pharmaceuticals, and biotechnology. It has the ability to hydrolyze 1,4-β-mannooligosaccharides.