Properties
Description
Recombinant Ruminococcus flavefaciens acetyl xylan esterase 15A2 produced by Escherichia coli, is responsible for the hydrolysis of the UDP-3-0-myristoyl-N-acetylglucosamine to transform UDP-3-O-myristoylglucosamine and acetate. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Ruminococcus flavefaciens
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminococcus flavefaciens acetyl xylan esterase 15A2 can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of acetylated xylan.