Properties
Description
Recombinant Ruminococcus flavefaciens acetyl xylan esterase 3B produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Ruminococcus flavefaciens
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminococcus flavefaciens acetyl xylan esterase 3B can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of α-naphthyl acetate, and β-naphthyl acetate.
