Properties
Description
Recombinant Ruminococcus flavefaciens carbohydrate-binding module Rf999 produced by Escherichia coli, with ligand specificity of 1,3-1,4-β-glucans, cellulosic carbohydrates, galactomannans, xyloglucan, is a type of enzyme protein produced in Escherichia coli. It has multiple modules that specifically bind to carbohydrate substrates in order to carry out their function.
Expression System
Escherichia coli
Species
Ruminococcus flavefaciens
Carbonhydrate Specificity
Galactose
Purity
≥90%, determined by SDS-PAGE.
Activity
Carbohydrate binding modules
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminococcus flavefaciens carbohydrate-binding module Rf999 can be used for glycobiology research.
