Properties
Description
Recombinant Ruminococcus flavefaciens mannanse 5A produced by Escherichia coli, is a single-domain enzyme belonging to glycoside hydrolase family 5 (GH5).
Expression System
Escherichia coli
Species
Ruminococcus flavefaciens
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Ruminococcus flavefaciens mannanse 5A can be used in biofuel production and carbohydrate modification. It has the ability to hydrolyze galactomannans, glucomannans, and mannans.
