Properties
Description
Recombinant Streptococcus pneumoniae β-acetylglucosaminidase 85A produced by Escherichia coli, is an enzyme that is responsible for hydrolyzing the diacetylchitobiosyl unit in glycopeptides and glycoproteins with the (Man(GlcNAc)(2))Asn structure, releasing the intact oligosaccharide except for one N-acetyl-D-glucosamine residue that remains attached to the protein.
Expression System
Escherichia coli
Species
Streptococcus pneumoniae
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetylglucosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Streptococcus pneumoniae β-acetylglucosaminidase 85A can be used for glycobiology research. It has the ability to hydrolyze 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (3F4NP-GlcNAc), chitobiose core of N-glycans.
