Properties
Description
Recombinant Streptococcus pneumoniae blood-group endo-β-galactosidase 98A produced by Escherichia coli, acts as an enzyme that hydrolyzes the 1,4-β-D-galactosyl linkages adjacent to a 1,3-α-D-galactosyl or N-acetylgalactosaminyl residues and a 1,2-α-D-fucosyl residue.
Expression System
Escherichia coli
Species
Streptococcus pneumoniae
Purity
≥90%, determined by SDS-PAGE.
Activity
β-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Streptococcus pneumoniae blood-group endo-β-galactosidase 98A can be used in various areas such as carbohydrate modification and glycan analysis. It has the ability to hydrolyze blood group A/B antigens.
