Properties
Description
Recombinant Thermobifida fusca carbohydrate-binding module 2F2 produced by Escherichia coli, with ligand specificity of 1,4-β-mannans, is a type of enzyme protein produced in Escherichia coli. It has multiple modules that specifically bind to carbohydrate substrates in order to carry out their function.
Expression System
Escherichia coli
Species
Thermobifida fusca
Carbonhydrate Specificity
1,3-β-Linked glucooligosaccharide, 1,4-β-linked glucooligosaccharide, 1,6-β-linked glucooligosaccharide, mannans, mannooligosaccharides
Purity
≥90%, determined by SDS-PAGE.
Activity
Carbohydrate binding modules
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Thermobifida fusca carbohydrate-binding module 2F2 can be used for glycobiology research.
