Properties
Description
Recombinant Thermobifida fusca cellulase 5B produced by Escherichia coli, possesses broad exo-1,3/1,6-β-glucanase specificity and endo-β-1,4-glucanase activity.
Expression System
Escherichia coli
Species
Thermobifida fusca
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Thermobifida fusca cellulase 5B can be used in research for textile, animal food, pharmaceutical, detergent, and paper processing industries. It has the ability to hydrolyze avicel and cellulosic carbohydrates.