Properties
Description
Recombinant Thermobifida fusca cellulase 9B produced by Escherichia coli, possesses broad exo-1,3/1,6-β-glucanase specificity and endo-β-1,4-glucanase activity.
Expression System
Escherichia coli
Species
Thermobifida fusca
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Thermobifida fusca cellulase 9B can be used in research for textile, animal food, pharmaceutical, detergent, and paper processing industries. It has the ability to hydrolyze 4-nitrophenyl-β-D-cellobioside, cellulosic carbohydrates, and swollen cellulose.