Properties
Description
Recombinant Thermococcus kodakarensis exo-β-glucosaminidase 35A produced by Escherichia coli, plays a role in the hydrolysis of chitosan or chitosan oligosaccharides by removing consecutive D-glucosamine residues from the non-reducing ends.
Expression System
Escherichia coli
Species
Thermococcus kodakarensis
Purity
≥90%, determined by SDS-PAGE.
Activity
Glucosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Thermococcus kodakarensis exo-β-glucosaminidase 35A can be used in industries such as biotechnology and pharmaceuticals. It has the ability to hydrolyze chitobiose, a nonreducing end of chitooligosaccharides.