Properties
Description
Recombinant Thermotoga maritima hexosaminidase 3A produced by Escherichia coli, plays a key role in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides.
Expression System
Escherichia coli
Species
Thermotoga maritima
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Thermotoga maritima hexosaminidase 3A can be used in ELISA, western blot, immunoprecipitation, and SDS-PAGE. It has the ability to hydrolyze 4-nitrophenyl di-N-acetyl β-D-chitobiose, 4-nitrophenyl N-acetyl β-D-glucosamine, 4-nitrophenyl tri-N-acetyl β-D-chitotriose, 4-nitrophenyl-β-D-glucose, chitooligosaccharides.
