Recombinant Vibrio sp. alginate lyase 7A

Synonym: VAlg7A; Poly(beta-D-mannuronate) lyase; Poly[(1,4)-beta-D-mannuronide] lyase

Enzyme Commission Number: 4.2.2.3

Product Size

0.5 mg; 1 mg; 1.5 mg; 2 mg

Price

Datasheet

MSDS

Properties

Description

Recombinant Vibrio sp. alginate lyase 7A produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism. It is produced in Escherichia coli.

Expression System

Escherichia coli

Species

Vibrio sp.

Concentration

0.5 mg/mL

Form

Liquid

Purity

≥90%, determined by SDS-PAGE.

Activity

Alginate lyases

Buffer

35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl₂, and 25% (v/v) glycerol

Applications

Recombinant Vibrio sp. alginate lyase 7A can be used as a biocatalyst for the saccharification of alginate and participates in the eliminative cleavage of sodium alginate.