Properties
Description
Recombinant Zobellia galactanivorans poly α-guluronate lyase 7A2 produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Zobellia galactanivorans
Purity
≥90%, determined by SDS-PAGE.
Activity
Poly-α-guluronate lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Zobellia galactanivorans poly α-guluronate lyase 7A2 can be used in enzymatic degradation of polysaccharides with α-guluronate groups and participates in the eliminative cleavage of sodium alginate.
