Properties
Description
Recombinant keratanase II from Bacillus circulans cleaves the sulfated N-acetylglucosaminidic β1-3 linkage to galactose included in keratan sulfate.
Unit Definition
One unit will produce 1 umol of reducing oligosaccharides from shark cartilage keratan sulfate polysaccharide per minute at pH 6.0 at 37°C.
Expression System
E. coli
Source
Bacillus circulans
Reaction Conditions
20 mM Sodium acetate, pH 6.0
Production Methods
Fermentation
Detection Method
A 50 ul mixture of recombinant keratanase II and shark cartilage keratan sulfate is incubated at 37°C for 15 min, and the released oligosaccharide reducing end is determined by a modified Somogyi-Nelson method.
Sensitivity
Heat sensitive
Form
Colorless to Almost colorlessclear liquid
Buffer
20 mM Sodium phosphate, pH 7.4
Applications
Recombinant keratanase II from Bacillus circulans can be used to detect keratin sulfate proteoglycan content.
