Robinia pseudoacacia Lectin (RPA) Production Service at CD BioGlyco

RPA is a protein derived from the R. pseudoacacia tree. This lectin has a high binding affinity to galactose residues, which allows it to recognize human blood group antigens. RPA is utilized in diverse scientific fields, including histology, cytology, and biochemical research. CD BioGlyco provides high-quality Lectin Production for basic research. In particular, we have extensive experience in Plant Lectin Production and provide clients with customized lectin production services.

RPA Extraction Service

The extraction of RPA typically involves a multi-step process that allows the lectin to be isolated from other compounds. First, the R. pseudoacacia seeds are ground into a powder. This powder is then usually soaked in a buffer solution to help dissolve the proteins. The complex mixture is then centrifuged, with the supernatant collected for purification.

Fig.1 RPA production service. (CD BioGlyco)

RPA Purification Service

For the purification of RPA, commonly used methods include salting out, gel filtration chromatography, and ion exchange chromatography. These methods collectively enable the purification of RPA to a high degree, suitable for research or other applications.

• Salting out utilizes differential precipitation of the protein to separate it from other soluble proteins.
• Gel filtration chromatography separates proteins based on their size, as they pass through a porous gel. Proteins that are larger pass through more quickly, whereas smaller proteins take longer as they get caught in the pores of the gel.
• Ion exchange chromatography separates proteins based on their charge where protein molecules with the same charge as the charged beads in the column will be repulsed and move more quickly through the column.

We also provide a high-quality Recombinant Lectin Production Service.

RPA Structure Characterization

• Through the use of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight is subsequently ascertained.
• Electrospray-ionization tandem mass spectrometry (ESI-MS) is utilized to establish the carbohydrate composition.
• Tandem mass spectrometry and methylation analysis are employed to pinpoint the glycosylation sites.

RPA Activity Assay

• Haemagglutination assay: To detect the haemagglutination activity.
• Lowry technique: This is employed to ascertain the protein concentration in the extracts.
• Bradford assay.

Applications

• RPA especially binds to N-acetylgalactosamine and thyroglobulin. It is used to separate glycoconjugates such as glycoproteins and polysaccharides from mixtures.
• Lectin microarray carrying RPA is used for glycan structure elucidation, helping researchers carry out protein glycosylation and cell surface glycosylation studies.
• This lectin is used to visualize the localization of specific saccharide moieties and the distribution of plasma membrane glycoproteins.

Advantages

• Extraction and purification from natural sources by chromatographic procedures produce a sizeable quantity of lectin.
• New and traditional technologies are combined with a variety of methods to choose from.
• Rigorous quality testing during lectin isolation, purification, and structural analysis.

Through years of continuous optimization and updating, CD BioGlyco offers a wide range of plant lectin production services, including some of the most widely used lectins in biotechnology. We are constantly researching to expand our lectin service area. For our exceptional RPA production services, please don't hesitate to contact us. Our extensive production experience will help you accelerate your research.

Reference:

1. Kalmykova, E.V.; et al. Growth and development of seedlings of Robinia pseudoacacia for the production of planting material in conditions of chestnut soils of south of the Russian Federation. Research on Crops. 2023, 24(2): 357-365.