Sambucus nigra Lectin (SNA) Production Service at CD BioGlyco

SNA is isolated from S. nigra that is composed of two ricin-related lectins. Its molecular weight is 140 kDa. SNA is a tetrameric glycoprotein. SNA I consists of an A-chain (enzymatic activity) and a B-chain (carbohydrate-binding activity). SNA II consists of a B-chain (carbohydrate-binding) only.

CD BioGlyco is a company that Produces Lectins for basic research such as anthelmintic and antitumor research. We have a wide range of Plant Lectin products, such as Abrus precatorius Lectin, Aegopodium podagraria Lectin, etc. We improve to increase the yield of lectins by using emerging technologies and optimization. In addition, we use various purification methods to increase the purity of our lectins.

Isolation and Purification

We use our expertise in plant lectin production to optimize the efficiency of SNA production. We have traditional precipitate technologies and several emerging production technologies to choose from. Our traditional precipitate techniques include organic solvent (liquid-liquid extraction), salt (ammonium sulfate), etc. Microwave-assisted extraction, ultrasound-assisted extraction, supercritical fluid extraction, and other emerging lectin extraction techniques are also available.

There are various methods for the purification of plant lectins. The purification method is also one of the key steps in obtaining high-quality SNA. Affinity Chromatography is a common method for SNA purification. We optimize this purification step.

SNA is supplied in a variety of forms with a wide range of markings available. Recombinant lectin has the advantages of scalable production and stable performance. We also provide Recombinant Lectin Production Services.

Fig.1 SNA production and characterization service. (CD BioGlyco)

Structure Characterization

• Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), size-exclusion chromatography, and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) are used to identify the fractions’ molecular weight.
• Methylation analysis and electrospray-ionization tandem mass spectrometry (ESI-MS) are used to determine the carbohydrate composition.
• Tandem mass spectrometry is used to analyze N-glycans.

Activity assay

• Bradford assay
• Haemagglutination assay

Applications

• Glycoprotein studies: The structures of glycoproteins are difficult to analyze because of the linked carbohydrates. Lectin microarray provides a solution to help researchers to determine the glycosylation characteristics of various biological samples.
• The carbohydrate-binding specificity: SNA is a tetrameric glycoprotein, which recognizes GalNAc or Neu5Ac (α2-6) Gal linkages in glycoconjugates.
• SNA tends to bind to silicic acid on the α-2,6 (and to a lesser extent α-2,3) linked terminal galactose. Galactose or lactose will also inhibit binding to some extent.
• This lectin has been used to distinguish and separate oligosaccharides and glycopeptides containing GalNAc/Neu5Ac (α2-6) Gal from isomeric α-2,3-sugars.
• SNA inhibits cell-free protein synthesis.

Advantages

• Extraction and purification of lectins from S. nigra seeds by affinity chromatography. We use an optimized method to produce large quantities of lectins.
• Strict quality control platform for lectin production.
• Combining new technology with traditional technology.

CD BioGlyco has been innovating and optimizing our lectin production services. In addition, we have improved our lectin structure testing process to serve our clients more comprehensively. We will continue to conduct research on lectin production and application and expand our service areas. Please feel free to contact us and we will provide you with excellent SNA production service and abundant products.

Reference:

1. Iglesias, R.; et al. Structure and biological properties of ribosome-inactivating proteins and lectins from elder (Sambucus nigra L.) Leaves. Toxins. 2022, 14(9): 611.