Sialyl-T Antigen Inhibitor Development Service at CD BioGlyco

It has shown that the sialyl-T antigen is not only expressed in normal cells but is also abnormally expressed in most human tumor cells and precancerous lesions. Therefore, the development of sialyl-T antigen inhibitors interferes with the biosynthesis as well as the expression process of sialyl-T antigens, which is important for human tumor and cancer research.

At CD BioGlyco, we provide sialyl-T antigen inhibitor development services that include but are not limited to:

• Synthesis of sialyl-T antibody
  • We utilize biological techniques to develop specific sialyl-T antibodies to inhibit the sialyl-T antigen.
  • We offer optimized mice hybridoma technology, whereby immune cells stimulated with sialyl-T antigen are differentiated into hybridoma cells, resulting in the production of either polyclonal or monoclonal antibodies to the sialyl-T antigen. This technology has the advantage of greatly improving antibody quality and success rates.
  • We offer recombinant antibody production technology for rapid high-throughput and large-scale production of sialyl-T antibodies. Our technology can produce purified antibodies in just a few weeks, from gene sequence to the final product.
• Sialyl-T antigen inhibitor development We utilize high-throughput screening methods to screen for biomolecules, such as enzymes or glycoproteins, involved in sialyl-T antigen synthesis and expression. Subsequently, we modify these screened biomolecules, which have been shown to inhibit sialyl-T antigen synthesis and expression.

In addition, we offer in vivo and in vitro testing in animals, as well as techniques such as attrition cytometry to detect the inhibitory effects of sialyl-T antibodies and sialyl-T antigen inhibitors, among other bioactivities.

Fig.1 Development process of sialyl-T antigen inhibitors. (CD BioGlyco)

Publication

• Technology: Chemoenzymatic labeling strategy to track sialyl-T antigen
• Journal: ACS Central Science
• IF: 18.2
• Published: 2018
• Results: This method exploits the loose donor specificity and stringent acceptor specificity of human salivary acyltransferase to visualize, quantify, and enrich sialyl-T by rapid in vitro labeling of sialyl-T with a triazolyl-linked biotin-functionalized salivary acid derivative.
  The target epitope is first directly biotinylated by a simple one-step reaction. After the labeling reaction, the biotin moiety is introduced, and the labeling efficiency of the differently labeled probes is examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting with streptavidin coupled. As shown, successful cell labeling requires both the enzyme and the labeling probe. Experiments showed that 100 μM of biotin moiety is sufficient for robust labeling. Furthermore, similar results were observed when another sialyl-T-positive cell line, colon cancer HT29, was labeled with the labeling probe described above. Indeed, even other truncated mucin-type O-glycans, such as T-antigen and Tn-antigen, can be detected using the above strategy as well.

Fig.2 (A) Structures of the reporter probes used for biotinylation 3 and 4. (B) Western blot analysis of chemoenzymatic labeling of breast cancer MCF7 and colon cancer HT29 cells. (Wen, et al., 2018)

Applications

• Sialyl-T antigen is synthesized from Tn antigen, and sialyl-T antigen inhibitors can be used to expand the structure of Tn antigen and to study the role of Tn antigen in tumor suppression.
• The development of sialyl-T antigen inhibitors can be used in research on tumors or cancers.

Advantages

• Our highly professional and experienced researchers provide custom sialyl-T antigen inhibitor development solutions to our clients.
• Our sialyl-T antigen inhibitors development services are high quality and efficient and help our clients solve most of the related challenges.

CD BioGlyco is dedicated to Glycosylation Inhibitor Development Services, and we offer many types of inhibitor production services in this area. We hope to be your right hand in glycobiology. If you are interested in our services, please feel free to contact us.