CD BioGlyco is dedicated to meeting all your needs in human milk oligosaccharides (HMOs) research. We provide high-quality HMO products, and HMO separation, production, and analytical services to support your innovative discovery.
Breast milk is a highly complex fluid with nutrient-balancing, anti-pathogenic, anti-infective, anti-inflammatory, and immunomodulatory properties. An important component of human milk is sialylated and fucosylated HMOs molecules, which is essential for the normal development and health of newborns. Sialylated HMOs in human milk can be recognized and bound by sialic acid-dependent pathogens and inhibit neonatal epithelial cell adhesion. Small amounts of intact sialylated HMOs can be absorbed from the gut and remain in the circulation of newborns in sufficiently high concentrations to regulate the immune system at the cellular level and promote normal brain development in infancy.
Sialyllacto-N-tetraose a (LST a) is a sialic acid oligosaccharide present in human breast milk, which has biological significance such as anti-pathogen adhesion and providing precursors for brain biosynthesis. The structure of LSTa is Neu5Acα2,3Galβ1, 3GlcNAcβ1,3Galβ1,4Glc. In industry, LST a can be sialylated with α(2,3)-sialyltransferase (PmST1) on lacto-N-tetraose (LNT) by using a one-pot, three-enzyme system.
Fig.1 Structure of LST a. (CD BioGlyco)
Mass spectrometry (MS) is one of the most sensitive and rapid methods for characterizing sialylated HMOs. Structural analysis of sialylated HMOs is generally more difficult than that of neutral HMOs due to the easy dissociation of sialic acid. Studies have found that combining high-performance liquid chromatography (HPLC) with MS can eliminate many difficulties in the analysis of sialylated oligosaccharides (OS). LC-MS provides composition and structure information during OS analysis, while LC-MS/MS provides efficient discrimination of heterogeneous species. In addition, constructing an OS library is also an emerging method for the rapid analysis of HMOs.
CD BioGlyco's experimental team has extensive experience in the analysis of HMOs. We have developed High-Performance Liquid Chromatography (HPLC), Mass Spectrometry (MS), and Capillary Electrophoresis (CE) for the analysis of HMOs in human or animal milk samples. High-performance anion exchange chromatography (HPAEC) with pulsed amperometric detector (PAD) is our most common method for analyzing sialylated HMOs such as LST a. We also offer method development services to meet your specific project needs.
In addition, we also provide diverse HMO products for global clients, including but not limited to:
| Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| XCO0089Q | Lacto-N-biose | XCO0101Q | Disialosyl galactosyl globoside |
| XCO0090Q | N-Acetyl-D-lactosamine | XCO0102Q | Lacto-N-tetraose |
| XCO0091Q | 2'-Fucosyllactose | XCO0103Q | Lacto-N-neotetraose |
| XCO0092Q | 3-Fucosyllactose | XCO0104Q | Lacto-N-fucopentaose I |
| XCO0093Q | Lactodifucotetraose | XCO0105Q | Lacto-N-fucopentaose III |
| XCO0094Q | Lacto-N-triose I | XCO0106Q | Disialyllacto-N-tetraose |
| XCO0095Q | Lacto-N-triose II | XCO0107Q | 3'-Sialyllacto-N-neotetraose |
| XCO0096Q | 3'-Sialyllactose sodium salt | XCO0108Q | Sialyllacto-N-tetraose a |
| XCO0097Q | 3'-Sialyl-N-acetyllactosamine sodium salt | XCO0109Q | Sialyllacto-N-tetraose b |
| XCO0098Q | 6'-Sialyllactose sodium salt | XCO0110Q | Sialyllacto-N-tetraose c |
| XCO0099Q | 6'-α-Sialyl-N-acetyllactosamine sodium salt | XCO0111Q | p-Lacto-N-neohexaose |
| XCO0100Q | 3'-Sialyl-3-fucosyllactose |
CD BioGlyco develops a One-stop Solution for HMO Production, providing various HMO production and analysis services. The developed HMO Profiling method is not only highly accurate but also reproducible. If you are interested in our LST a analysis service, please feel free to contact us for more information.
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