As a leading glycobiology company, CD BioGlyco provides synthesis, analysis, and characterization services of sugar-related substances relying on advanced technology and an experienced research team.

Significance of Sialylated Human Milk Oligosaccharides (HMOs)

Human milk is a rich source of sialylated structures such as HMOs, glycoconjugates such as glycoproteins and glycolipids, and also contains sialic acid as a free molecule. Sialylated HMOs play an important role in the growth and development of infants: 1) Sialylated glycans of breast milk are key components of important gangliosides and nerve cell adhesion molecules in neonatal and infant neurodevelopment; 2) Sialylated glycans of breast milk are involved in inhibiting the adhesion of pathogens to host epithelial cells, significantly reducing the risk of intestinal diseases and ear and respiratory tract infections in infants; 3) Sialylated HMOs are involved in regulating the glycosylation pattern on the surface of infant epithelial cells, inducing the expression of related genes; 4) In the inflammatory process, sialylated glycans of breast milk are essential for leukocyte extravasation and mucosal infiltration. Sialylated HMOs have attracted increasing attention and their structures are usually very complex. Therefore, the development of fast and sensitive methods is crucial to elucidate structures and study biological functions of sialylated HMOs.

Fig.1 Inhibition of pathogen adhesion to epithelial cells of infant's gastrointestinal tract by sialylated oligosaccharides of human milk. (Lis-Kuberka & Orczyk-Pawiłowicz, 2019)

In recent years, many analytical techniques have been applied to analyze sialylated HMOs, such as mass spectrometry (MS), liquid chromatography (LC), and capillary electrophoresis (CE). Although these methods allow higher UV or fluorescence detection, they do not provide specific structural information. Therefore, the combination of LC and MS has been widely and successfully used for the analysis of sialylated HMOs in various milk samples.

Sialyllacto-N-tetraose b (LST b) Analysis Service at CD BioGlyco

LST b is a sialylated oligosaccharide present in human breast milk, and its sialic acid residues are linked to N-acetylglucosamine (GlcNAc) via an α2,6-glycosidic bond. The structure of LST b is Galβ1, 3(Neu5Acα2,3)GlcNAcβ1,3Galβ1,4Glc. CD BioGlyco has developed a rapid and sensitive LST b assay method to analyze the content and structure of LST b in human milk and animal milk samples. For special samples, we also provide customized method development services to meet customer needs.

Fig.2 Structure of LST b. (CD BioGlyco)

In addition, we also provide diverse HMO products for global clients, including but not limited to:

Advantages of Us

• Rapid and sensitive LST b assay method
• Suitable for human and animal milk samples
• Customized method development service
• Professional technical team to track the project throughout the process

CD BioGlyco's one-stop HMO solution is committed to providing comprehensive, reliable, and accurate production and analysis services for enterprises, universities, and research institutes. We sincerely hope to cooperate with you and help with your scientific research work. Please feel free to contact us for more information.

References:

1. Lis-Kuberka, J.; Orczyk-Pawiłowicz, M. Sialylated oligosaccharides and glycoconjugates of human milk. The impact on infant and newborn protection, development and well-being. Nutrients. 2019, 11(2): 306.
2. Wu, S.; et al. Annotation and structural analysis of sialylated human milk oligosaccharides. Journal of proteome research. 2011, 10(2): 856-868.