CD BioGlyco provides an integrated One-stop Solution for HMO Research. Our advanced technology platforms and high-quality services will fully meet your needs. We have the confidence to be your essential research assistant in the field of glycobiology.
HMOs, contribute to several important biological effects. These oligosaccharides reach the colon, where they act as prebiotics and stimulate the growth of beneficial bacteria, thereby affecting the composition of healthy gut flora. In addition, HMOs can modulate immune function by altering host epithelial and immune cell responses and help maintain a balanced inflammatory response. Several studies have shown that HMOs protect against pathogens such as Vibrio cholerae, Streptococcus pneumoniae, enteropathogenic E. coli, Campylobacter jejuni, and even HIV. Furthermore, HMOs acting as decoys, that is, binding to pathogens to inhibit bacteria and viruses from adhering to cell surface receptors, apparently protect breastfed infants from infection. In addition to their prebiotic, immunomodulatory, and anti-adhesion effects, HMOs may also affect cognitive function in newborns.
LSTc is a sialylated HMO. Sialylated oligosaccharides exist in various body fluids in the form of free oligosaccharides. Sialylated oligosaccharides have multiple functions in the body. The binding of sialic acid-containing glycans pathogens to host mucosal surfaces may be inhibited by human milk sialyloligosaccharides. Sialyloligosaccharides in human milk have also been shown to provide precursors for brain biosynthesis.
Fig.1 Chemical structure of LSTc. (PubChem)
CD BioGlyco provides a variety of available techniques for the identification and quantification of LSTc. Separation can be performed by liquid chromatography using hydrophilic interaction (HILIC), graphitized carbon or anion exchange (HPAEC) columns, or by electric field-mediated separation techniques such as capillary electrophoresis (CE) or microchip electrophoresis (ME). Liquid chromatography can be coupled with detection systems such as evaporative light scattering (ELSD), mass spectrometry (MS), refractive index (RI), fluorescence (FL), ultraviolet (UV), pulsed amperometric (PAD), or charged aerosol detection (CAD). MS, CAD, ELSD, and RI can detect oligosaccharides without labeling. The latter two detectors are limited by the use of gradients that are less sensitive than MS and CAD. For fluorescence (FL) or UV detection, a derivatization or labeling step is necessary because most HMOs are non-UV or fluorescence active.
Using the HPLC method as an example, our workflow is shown in the figure below.
Fig.2 The workflow diagram for LSTc analysis by HPLC. (CD BioGlyco)
CD BioGlyco provides an integrated solution for HMO Profiling. We provide LSTc analysis by various advanced methods such as HPLC for clients. If you are interested in our services, please contact us for more details without any hesitation.
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