Sorbose Analysis Service

Functions of Sorbose

Sorbose, a monosaccharide ketose, is usually in the form of a pyran-type ring. Sorbose is mainly synthesized by microbial fermentation method and has an extremely important physiological role and application value, widely used in pharmaceutical, cosmetic, food, and other industries. Further study of the structure-function relationship of sugar chains will accelerate the development of glycobiology. As a new target for drug development for hypertension, hyperglycemia, hyperlipidemia, and other diseases, a comprehensive analysis of sorbose is necessary for a better in-depth study of its conformational relationship.

Fig.1 α-L-sorbose, and their three conformer classes generated by the rotation of the exocyclic CH₂OH group. (Palivec, et al., 2020)

Sorbose Analysis Service at CD BioGlyco

Since sorbose and sorbitol are difficult to separate, rigorous separation and characterization techniques need to be developed. We use a variety of large-scale instruments to provide you with the most convenient and fastest analysis service.

• Sorbose monitoring by Fourier transfer infrared spectroscopy (FTIR) provides a wealth of chemical information due to the fundamental vibrations of the molecule.
• For qualitative analysis, the use of gas chromatography-mass spectrometry (GC-MS) in combination provides high separation efficiency and molecular weight information in a single analysis.
• Our company uses Raman optical activity (ROA) to analyze the structure of sugar in aqueous solution. Using molecular dynamics and density functional theory to explain the experimental Raman and reaction spectra of sorbose obtained in the extended range (50-4000 cm^-1), focusing on the CH stretching mode.

Fig.2 The flowchart of psicose analysis. (CD BioGlyco)

Publication

Paper Title: Simultaneous quantitative analysis of sorbitol and sorbose in the fermentation broth with Fourier transform infrared spectroscopy

Technology: FTIR

Journal: Applied Spectroscopy

IF: 3.5

Published: 2001

Results: This paper demonstrates a rapid and noninvasive method to simultaneously monitor sorbitol and sorbose concentrations in broth. The infrared spectra of sorbitol and sorbose in the aqueous solutions are shown in Fig.3. The relevant absorbance bands are situated at 1416, 1081, and 1047 cm^-1 for sorbitol and 1160, 1108, and 1052 cm^-1 for sorbose, respectively. There is a prominent sorbitol peak at 1081 cm^-1 and a sorbose peak at 1052 cm^-1, which are relatively isolated to be ideal candidates for quantitative analysis. Attenuated full repeat FTIR is an effective technique for both qualitative and quantitative monitoring of biological processes.

Fig.3 Infrared spectra of sorbitol and sorbose. (Qiu, et al., 2001)

Applications

• Development of lowering blood lipid and blood glucose drugs, a research target for the treatment of diabetic diseases.
• Sorbose is also used in animal and microbial metabolism research.
• This method is further used to study the control and optimization of metabolism, reaction kinetics, and biological processes.

CD BioGlyco attaches great importance to the analysis of Carbohydrate Metabolism. Moreover, we provide efficient Disaccharide Analysis Services, Oligosaccharide Analysis Services, and Polysaccharide Analysis Services. please feel free to contact us.

References:

1. Palivec, V.; et al. Raman optical activity of glucose and sorbose in extended wavenumber range. Chemphyschem. 2020, 21(12): 1272-1279.
2. Qiu, J.; et al. Simultaneous quantitative analysis of sorbitol and sorbose in the fermentation broth with Fourier transform infrared spectroscopy. Applied Spectroscopy. 2001, 55(6): 794-798.