Functions of Stachyose

Stachyose is a disaccharide sugar, which consists of two fructose molecules linked through a glycosidic bond. It occurs naturally in certain plants like soybeans, asparagus, and chicory. Studies have shown that stachyose, as an intestinal microecological regulator, can improve gastrointestinal function and increase water-soluble dietary fiber.

Fig.1 Functional oligosaccharide fermentation in the gut. (Zhang, et al., 2022)

Stachyose Analysis Service at CD BioGlyco

CD BioGlyco provides various oligosaccharide analysis services to meet the specific requirements of our global clients. Our analysis methods include high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), and fluorophore-assisted carbohydrate electrophoresis (FACE), electrospray Ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS).

• HPAEC-PAD

HPAEC-PAD is mainly used for stachyose analysis in samples containing various carbohydrates.

• CE

CE is applied to the analysis of various carbohydrates from glycoconjugates, which is one of the most powerful techniques in terms of resolving power.

• HPLC

HPLC is at present the main analytical technique used for carbohydrates analysis.

• FACE

FACE is applied to determine the presence and relative abundance of individual oligosaccharides in a mixture of oligosaccharides at a simple and sensitive method.

• ESI-MS, MALDI-TOF-MS

These analytical techniques are applied for the analysis of oligosaccharides and have been widely used in the analysis of mixtures.

Our analysis process is as follows:

• Sample preparation: Before analysis, the samples were mixed softly to guarantee homogenization and then directly weighted.
• Extraction and fractionation: We use filtration and supercritical fluid extraction for extraction and fractionation.
• Chemical treatments: We treat the samples by hydrolysis and derivatization. Frequently, before chromatographic analysis, a hydrolysis step is carried out of large carbohydrates to obtain fractions of low molecular mono- or oligosaccharides that can be analyzed more easily.
• Qualitative and quantitative analyses: HPLC is used for stachyose analysis.

Fig.2 Flowchart of stachyose analysis. (CD BioGlyco)

Applications

• We analyze stachyose content in functional foods to better monitor product quality.
• Stachyose is highly viscous and highly hygroscopic, monitoring stachyose in foods to prevent them from drying out.
• Stachyose cannot be digested by the human body and does not produce heat, so it is used in the development of food for diabetic patients.

Advantages

• Multiple technologies for choice
• High accuracy and efficiency
• Targeted customized service plan
• Perfect after-sale service

CD BioGlyco is dedicated to providing accurate, reliable, and reproducible stachyose analysis services for our clients worldwide. Besides, we also offer customized solutions in Oligosaccharide Analysis Services according to your specific needs. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of Carbohydrate Metabolism analysis.

Reference:

1. Zhang, N.; et al. Functional oligosaccharide fermentation in the gut: improving intestinal health and its determinant factors-a review. Carbohydrate Polymers. 2022, 284: 119043.