Unveiling the Sweet Science: Steviol Glycoside Analysis Excellence

Steviol glycoside is a kind of glycoside extracted from the leaves of Stevia rebaudiana, the main component is stevioside (C₃₈H₆₀O₁₈), usually accompanied by rebaudioside A, B, C, D, F, dulcoside A, sweet tea glycoside and stevia disaccharide glycoside and other glycoside components. Steviol glycoside is often used as a new natural sweetener, and also steviol glycoside is a pharmaceutical excipient with anti-diabetic, antihypertensive, anti-inflammatory, antibacterial, and other functions.

As a company specializing in glycobiological research, CD BioGlyco has accumulated extensive experience in the field of Pharmaceutical Excipient Analysis and provides custom steviol glycoside analysis services to clients.

• Identification
  Thin layer chromatography: The sample and the standard of steviol glycoside are dissolved in anhydrous ethanol, and then spotted on the same silica gel thin layer plate and unfolded. By comparing the spot positions of the sample and the standard, it can be identified whether the sample is steviol glycoside or not.
• Properties
  Steviol glycoside is a white or off-white crystal or powder, soluble in a mixture of ethanol and water.
• Specific rotation
  The specific rotation of steviol glycoside is determined after it has been fully dissolved in a mixture of ethanol and water and should be between -30° and -40°.
• Acidity
  The acidity of steviol glycoside can be measured using a pH meter. The pH of steviol glycoside should be 4.5 to 7.0.
• Content assay
  Titration: We titrate a sample solution of steviol glycoside using phenolphthalein as an indicator solution and an alcoholic solution of potassium hydroxide as a titrant. The titration is stopped when the solution turns red. The content of steviol glycoside (C₃₈H₆₀O₁₈) in the sample can be calculated from the amount of titrant used.
• Solvents (methanol and ethanol)
  Liquid chromatography: We measure methanol and ethanol in steviol glycoside by liquid chromatography method which uses polyethylene glycol as a stationary solution and butanol as an internal standard solution. The amount of methanol and ethanol can be calculated from the internal standard method by peak area.
• Loss on drying
  At higher temperatures, we dry steviol glycoside. After complete drying, the weight loss of steviol glycoside should not exceed 5.0% of the weight of the dried product of steviol glycoside.
• Scorch residue
  The amount of residue remaining after scorching of steviol glycoside is not to exceed 0.1 percent.
• Heavy metals
  The residue from the burning of steviol glycoside is treated with acid, to which acetate buffer and thioacetamide test solution are added. If the steviol glycoside contains heavy metal impurities, the whole solution becomes darker in color.
• Lead
  The amount of lead contained in steviol glycoside can be calculated from the absorbance at 283.3 nm, which is determined by atomic absorption spectrophotometry, provided that steviol glycoside is completely digested.

Advantages

• We offer custom steviol glycoside analysis services to meet the specific needs of different clients.
• We provide in-depth interpretation and analysis of steviol glycoside analysis results, provide clear and comprehensive data reports, and offer professional advice to our clients.
• We follow strict laboratory practices and standards to ensure the accuracy and reliability of our steviol glycoside analysis results, as well as compliance with relevant regulations and standards.

CD BioGlyco offers a custom Pharmaceutical and Biological Analysis Service to meet the needs of our clients. Besides, we also provide our clients with relevant consultancy and professional support on steviol glycoside to help them better understand and apply the analysis results. Please feel free to contact us for more details if you are interested in our services.