Properties
Description
Sucrase from yeast, a digestive enzyme, is responsible to catalyze the hydrolysis of sucrose to its subunits fructose and glucose. β-Galactosidase from Aspergillus niger, a crucial enzyme in the human body, is responsible for hydrolyzing galactosides into galactose.
Molecular Weight
57.7 kDa
Unit Definition
One unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium phosphate buffer (100 mM), pH 6.8 at 40°C.
β-Galactosidase from Aspergillus niger: One unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40°C.
Source
Sucrase: yeast; β-Galactosidase: Aspergillus niger
Applications
This product can be used for the separation of sucrose in the cereals, food and feeds, fermentation, and beverage industries.
