Trichinella spiralis Antigen Production Service
Specific Antigen of Trichinella spiralis
T. spiralis causes trichinellosis, which is usually transmitted to humans or other animals through the consumption of infected meat. The epitope of the 18H1 antibody contains the tri-antennary and tetra-antennary N-glycans of tyvelose, which are unique structures to T. spiralis. 18H1 binds to tyvelose (3,6-dideoxy-D-arabinohexose) on secreted glycoproteins and surface glycoproteins. The antigens carrying tyvelose are called TSL-1, which are produced in the needle cell granules in the needle bodies of the larvae and induce a strong antibody response in parasitic animals. TSL-1 antigens are modulators of the immune system and display high immunogenicity due to hydrophobic oligosaccharide chains with repeated tyvelose and fucose.
T. spiralis Antigen Production Service at CD BioGlyco
- Antigen acquisition
CD BioGlyco uses animal models known to be infected with T. spiralis or artificially cultured cells to produce anti-T. spiralis 18H1 antibodies through hybridoma technology and phage display technology respectively, and incubate them. We separate the cultured parasites from the culture fluid or tissue and extract the antigenic substances through cell disruption, sonication, or other methods.
- Antigen purification
CD BioGlyco uses different techniques (such as centrifugation, electrophoresis, and chromatography) to purify the extracted antigens to remove impurities and other non-target components.
- Antigen characterization
CD BioGlyco performs characterization of the produced antigens, including immunogenicity assessment, immunological testing, and characterization of the antigens.
Vaccine Development Service at CD BioGlyco
CD BioGlyco has established a powerful Carbohydrate-based Adjuvant Development platform, and we provide vaccine formulation development services based on the T. spiralis antigen. We select appropriate adjuvants, vectors, or expression systems while optimizing the combination and concentration of the antigen to achieve the best immune response and protective effect. We also provide immunological evaluation services to evaluate the immunogenicity and protective efficacy of candidate vaccines through experiments such as animal model immunity tests, antibody response analysis, and cellular immunological assays.
Fig.1 T. spiralisantigen production and vaccine formulation development services. (CD BioGlyco)
Applications
- Development reagents: T. spiralis antigen is applied to develop reagents for T. spiralis infection, such as ELISA kits, immunochromatographic test strips, and molecular diagnostic reagents, which are applied to detect the presence of infection.
- Vaccine development: Produced T. spiralis antigen is used as active ingredients in vaccine candidates for the design and optimization of vaccines as preventive against Trichinella infection.
- Drug screening: T. spiralis antigens are utilized to screen and assess the efficacy of new antiparasitic drugs, which are used to evaluate the inhibitory effect of drug candidates against T. spiralis infection.
Advantages
- CD BioGlyco has a mature antigen production process, that produces T. spiralis antigen preparations with high quality, high purity, and stable activity.
- CD BioGlyco provides personalized T. spiralis antigen production services based on client needs, including selecting antigens for specific stages and adjusting the degree of purification and concentration.
- CD BioGlyco provides a continuous and stable supply of T. spiralis antigens to meet clients' long-term research or product development needs.
CD BioGlyco is the world's top biotechnology service company and has a powerful Glyco™ Vaccine Development Platform. We constantly pursue technological innovation and process improvement to provide better quality T. spiralis antigen production and vaccine development services. Please feel free to contact us if you would like to inquire about details.
References
- Reason, A.J.; et al. Novel tyvelose-containing tri-and tetra-antennary N-glycans in the immunodominant antigens of the intracellular parasite Trichinella spiralis. Glycobiology. 1994, 4(5): 593-603.
- Braasch, J.; et al. Trichinella spiralis–new method for sample preparation and objective detection of specific antigens using a chemiluminescence immunoassay. Veterinary Parasitology. 2020, 277, 100033.
