Tailoring Cell Membranes with Biologically Active Glycans for Clients

Glycosaminoglycans (GAGs) are a class of sulfated linear polysaccharides. Its sulfation pattern interacts with various proteins and is involved in processes such as cell growth and development, cancer metastasis, and neuronal growth. Based on De Novo Glycan Display, CD BioGlyco intervenes in normal physiological functions and pathways of disease onset by modulating its different sulfation patterns. We help our clients investigate the conformational relationships and in vivo effects of specific sulfated motifs by deciphering the complex structure and modifying the roles of cell surface GAG.

At CD BioGlyco, we synthesize a lipid and modify a specific glycan (e.g., chondroitin sulfate (CS)) on the cell surface. We provide a simple and efficient pathway to tailor membranes with bioactive glycans and study key cellular events by cell surface glycan engineering.

• Liposome synthesis and validation strategy

Our researchers use liposomes as glycan carriers for cell surface display of biomolecules. We use two main lipids such as 1,2-dioleoyl-3-trimethylpropanaminium chloride (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE). Labeling of different cell types is achieved by lipid-functionalized fluorophores of DOPE-based liposomes. Then, we use oxime chemistry to add glycans. Key to this strategy is the need for free radical-mediated synthesis of polymers for lipid and glycan functionalization.

We optimize the membrane fusion ratio to ensure the best ratio. Meanwhile, we provide the relative level test service of cell surface fluorophore incorporation by fluorescence-assisted cell sorting (FACS). In addition, to characterize biophysical properties, we provide transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential measurement services.

• Cellular events

We monitor the signaling pathways for liposome-mediated cell surface-specific and polysaccharide-modified cell activation. Liposomes pre-functionalized with polysaccharides are incubated and immunostaining with antibodies, then we image the process using confocal fluorescence microscopy. Moreover, we offer membrane lifetime monitoring of exogenous lipids.

Fig.1 Strategies to remodel cell surfaces with glycans. (CD BioGlyco)

Publication

Applications

• Custom bioactive glycans to modify cell membranes extend the various glycans with intercellular conduction signal analysis and functional impact assays.
• Custom bioactive glycan-modified cell membranes are widely used in many important biological environments such as neurodegeneration, stem cell differentiation, and cancer.
• Custom bioactive glycan-modified cell membranes are new research targets for glycan engineering to regulate complex cells, providing a useful tool for biomolecules to reshape cell membranes.

Advantages of Us

• The lipid carriers we provide have biophysical properties that are easily prepared, have low cytotoxicity, and are adjustable.
• With the help of a professional Glycan Display platform, we provide a comprehensive system to provide clients with high-quality services.

CD BioGlyco has efficient synthesis staff and experienced staff to help clients achieve their goals. We are committed to being your best partner in the de novo glycan display. If you are interested in our service, please feel free to contact us for a quote and more information.